噬菌体Bp7耐受株K12-R生物学特性分析

doi:10.11843/j.issn.0366-6964.2018.02.019

畜牧兽医学报 ›› 2018, Vol. 49 ›› Issue (2): 396-400.doi: 10.11843/j.issn.0366-6964.2018.02.019

噬菌体Bp7耐受株K12-R生物学特性分析

陈培培, 齐心, 王薇, 任慧英, 刘文华, 张灿*

青岛农业大学动物医学院, 青岛 266109

收稿日期:2017-07-07 出版日期:2018-02-23 发布日期:2018-02-23
通讯作者: 张灿,副教授,E-mail:cleverflame@163.com
作者简介:陈培培(1992-),女,山东菏泽人,硕士生,主要从事微生物与免疫研究,E-mail:1178968687@qq.com
基金资助:
国家自然科学基金（31600149）

Biological Analysis of a Phage Bp7 Resistant Strain K12-R

CHEN Pei-pei, QI Xin, WANG Wei, REN Hui-ying, LIU Wen-hua, ZHANG Can*

College of Veterinary Science, Qingdao Agricultural University, Qingdao 266109, China

Received:2017-07-07 Online:2018-02-23 Published:2018-02-23

摘要/Abstract

摘要：

宿主菌E.coli K12的突变株K12-R不能被噬菌体Bp7裂解，为了明确其产生耐受的原因以及突变对其生物学性能的影响，对K12-R进行全基因组测序，分析其产生耐受的原因，浊度测定K12-R的生长曲线和自凝能力，革兰染色检测K12-R生物膜的形成能力，比较K12-R和E.coli K12之间生物学性能的变化。结果表明，突变株K12-R脂多糖合成相关基因hldE发生了突变，突变株K12-R细胞膜通透性增加，生长繁殖能力降低，自凝能力增强，生物膜形成能力增强。E.coli K12的脂多糖合成基因hldE突变能够改变K12-R脂多糖的结构，从而抑制噬菌体Bp7的吸附。这为明确K12-R突变株对噬菌体Bp7的耐受机制研究提供了理论依据。

Abstract:

E. coli K12 was the host of phage Bp7, and its mutant strain K12-R was resistant to phage Bp7. To understand the resistance reason and the mutation effect on biological properties of K12-R, the genome of K12-R was sequenced and compared with that of E. coli K12 to find the mutation sites, the growth ability and the autoaggregation ability of K12-R were measured by turbidity test and the biofilm formation ability was detected by gram staining method, the biological properties of K12-R were compared with E. coli K12. The results showed that a mutant gene hldE, which was associated with Lipopolysaccharide synthesis, in K12-R caused the resistance to phage Bp7. Compared with E. coli K12, the growth ability of K12-R was reduced, but the cell membrane permeability, the autoaggregation ability and the biofilm formation ability were enhanced. The hldE gene of E. coli K12 was associated with LPS synthesis, the mutation of hldE gene changed the LPS character of K12-R, which caused the resistance to phage Bp7. The results provides the basis to understand the tolerance mechanism of K12-R to phage Bp7.

中图分类号:

S852.6

陈培培, 齐心, 王薇, 任慧英, 刘文华, 张灿. 噬菌体Bp7耐受株K12-R生物学特性分析[J]. 畜牧兽医学报, 2018, 49(2): 396-400.

CHEN Pei-pei, QI Xin, WANG Wei, REN Hui-ying, LIU Wen-hua, ZHANG Can. Biological Analysis of a Phage Bp7 Resistant Strain K12-R[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(2): 396-400.

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噬菌体Bp7耐受株K12-R生物学特性分析

Biological Analysis of a Phage Bp7 Resistant Strain K12-R

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